《植物生理学报》 2012, 48(2): 141-146

6-BA、NAA和2,4-D不同配比对荠菜愈伤组织诱导、生长及植株再生的

李文静¹, 李学强^1,*, 贾毛毛², 唐洪梅¹, 宋乾江¹, 连少英¹

1河南科技大学林学院, 河南洛阳471003; 2华中农业大学园艺林学学院, 武汉430070

通信作者：李学强；E-mail: gnaiqeuxil@l63.com；Tel: 0379-64282669

摘　要：

以野生荠菜[Capsella bursa-pastoris (L.) Medic]无菌苗为试材, 选取下胚轴、子叶、真叶和叶柄作为外植体, 研究了不同外植体的出愈情况, 不同植物生长调节剂及配比对愈伤组织诱导、继代及植株再生的影响。结果表明: (1)下胚轴作为外植体出愈情况最好, 继代后生长快; (2)下胚轴愈伤组织的最适植株再生培养基为MS+2~3 mg·L-1 6-BA+0.2~0.6 mg·L-1NAA; (3)愈伤组织培养阶段的2,4-D浓度对其植株再生能力有影响。

关键词：荠菜; 愈伤组织; 植株再生; 2,4-D

收稿：2011-12-08   修定：2012-01-12

资助：河南科技大学大学生研究训练计划项目(SRTP2009113)。

Effects of 6-BA, NAA and 2,4-D on Callus Induction, Growth and Plantlet Regeneration of Shepherd’s Purse [Capsella bursa-pastoris (L.) Medic]

LI Wen-Jing¹, LI Xue-Qiang^1,*, JIA Mao-Mao², TANG Hong-Mei¹, SONG Qian-Jiang¹, LIAN Shao-Ying¹

¹Forestry College of Henan University of Science and Technology, Luoyang, Henan 471003, China; ²College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China

Corresponding author: LI Xue-Qiang; E-mail: gnaiqeuxil@l63.com; Tel: 0379-64282669

Abstract:

The test material is the germ-free plants of shepherd’s purse [Capsella bursa-pastoris (L.) Medic]. Using the hypocotyls, the cotyledons, the leaves and the petioles as explants, the appearance of different explants on callus induction and the effects of the different medium on callus induction, callus subculture and plant regeneration were investigated. The results showed that: (1) the hypocotyl was the best explant for callus induction and subculture; (2) the favorable medium of plant regeneration for the hypocotyls was MS+2-3 mg·L^-16-BA+0.2-0.6 mg·L^-1 NAA; (3) the concentration of 2,4-D that was used in callus growing had obvious effect on the callus redifferentiation.

Key words: shepherd’s purse [Capsella bursa-pastoris (L.) Medic]; callus; plantlet regeneration; 2,4-D